Below is a list of transit agencies in Florida. Ridership and Revenue Miles data is from the 2020 National Transit Database. Click on an agency’s name to go to the agency’s website.
| Agency | City | County | Fixed Route Ridership | Fixed Route Revenue Miles | Demand Response Ridership |
| Bay Town Trolley | Panama City | Bay | 621,365 (2017) | 496,483 (2017) | 94,999 (2017) |
| Broward County Transit | Plantation | Broward | 27,796,289 | 15,052,388 | 845,386 |
| Charlotte County Transit Division | Punta Gorda | Charlotte | 6,708 | 46,244 | 127,846 |
| Citrus County Transit | Lecanto | Citrus | 47,189 | 219,761 | 22,886 |
| Collier Area Transit (CAT) | Naples | Collier | 840,961 | 1,268,696 | 106,256 |
| Escambia County Area Transit (ECAT) | Pensacola | Escambia | 1,445,102 | 1,596,032 | 101,671 |
| Flagler County Public Transportation | Bunnell | Flagler | n/a | n/a | 109,590 |
| Gainesville Regional Transit System (RTS) | Gainesville | Alachua | 9,344,324 | 3,926,905 | 61,527 |
| Hernando (Trans-Hernando Express) | Brooksville | Hernando | 127,072 | 409,107 | 14,219 |
| Hillsborough Area Regional Transit Authority (HART) | Tampa | Hillsborough | 12,010,636 | 7,792,617 | 172,054 |
| Indian River (GoLine) | Vero Beach | Indian River | 1,220,339 | 978,431 | 32,501 |
| Jacksonville Transportation Authority (JTA) | Jacksonville | Duval | 11,718,575 | 9,182,314 | 369,347 |
| Lake County Public Transportation (LakeXpress) | Tavares | Lake | 340,650 | 571,150 | 107,312 |
| Lakeland Area Mass Transit District (Citrus Connection) | Lakeland | Polk | 1,144,300 | 1,457,881 | 108,299 |
| Lee County Transit (LeeTran) | Fort Myers | Lee | 3,192,840 | 3,390,026 | 133,765 |
| LYNX Transit | Orlando | Orange, Osceola, and Seminole | 24,637,967 | 18,272,240 | 479,954 |
| Manatee County Area Transit (MCAT) | Sarasota | Manatee | 1,368,460 | 1,336,838 | 97,528 |
| Martin County (MARTY) | Stuart | Martin | 83,467 | 494,181 | 6,821 |
| Miami-Dade Transit | Miami | Dade | 80,197,205 | 33,463,419 | 1,743,023 |
| Okaloosa County (EC Rider) | Fort Walton Beach | Okaloosa | 119,658 | 342,113 | 97,476 |
| Palm Tran | West Palm Beach | Palm Beach | 9,113,767 | 7,310,660 | 999,260 |
| Pasco County Public Transportation (PCPT) | Port Richey | Pasco | 826,811 | 1,649,879 | 58,495 |
| Pinellas Suncoast Transit Authority (PSTA) | St. Petersburg | Pinellas | 11,521,351 | 9,140,825 | 367,642 |
| St. Johns COA (Sunshine Bus) | St. Augustine | St. Johns | 300,165 | 632,358 | 55,573 |
| St. Lucie COA (Treasure Coast Connector) | Fort Pierce | St. Lucie | 434,198 | 521,386 | 102,979 |
| Sarasota County Area Transit (SCAT) | Sarasota | Sarasota | 2,410,225 | 3,067,498 | 195,061 |
| South Florida Regional Transportation Authority (Tri-Rail) | Pompano Beach | Broward, Miami-Dade, and Palm Beach | 5,217,786 | 1,808,500 | n/a |
| Space Coast Area Transit (SCAT) | Cocoa | Brevard | 2,205,014 | 2,505,383 | 423,209 |
| StarMetro (Tallahassee) | Tallahassee | Leon | 3,316,766 | 2,238,742 | 103,201 |
| SunRail (Central Florida Commuter Rail) | Sanford | Orange & Volusia | 831,460 | 281,561 | n/a |
| SunTran | Ocala | Marion | 416,242 | 484,964 | 14,725 |
| VOTRAN | South Daytona | Volusia | 3,214,960 | 3,147,083 | 314,019 |