Below is a list of transit agencies in Florida. Ridership and Revenue Miles data is from the 2022 National Transit Database. Click on an agency’s name to go to the agency’s website.
| Agency | City | County | Fixed-Route Passenger Trips | Demand-Response Trips | Fixed-Route Revenue Miles |
| Bay County (Bayway Transit) | Panama City | Bay | 303,829 | 45,452 | 1,478,881 |
| Broward County Office of Transportation | Plantation | Broward | 21,159,241 | 968,071 | 2,851,565 |
| Central Florida Commuter Rail (SunRail) | Sanford | Orange & Volusia | 868,700 | n/a | 1,790,750 |
| Central Florida Regional Transportation Authority (LYNX) | Orlando | Orange, Osceola, and Seminole | 16,170,764 | 560,468 | 2,044,964 |
| Charlotte County | Punta Gorda | Charlotte | n/a | 68,825 | n/a |
| Citrus County (reduced reporter) | Lecanto | Citrus | 15,449 | 23,883 | 1,381,925 |
| City of Ocala (SunTran) (reduced reporter) | Ocala | Marion | 189,289 | 15,458 | 3,295,298 |
| City of Tallahassee (StarMetro) | Tallahassee | Leon | 2,286,178 | 114,655 | 7,924,284 |
| Collier County | Naples | Collier | 662,396 | 83,492 | n/a |
| County of Volusia dba VOTRAN | South Daytona | Volusia | 2,072,039 | 238,021 | 659,686 |
| Escambia County Area Transit | Pensacola | Escambia | 733,141 | 109,590 | 1,966,644 |
| FDOT D1 Vanpools (Commute Connector) (reduced reporter) | 330,512 | n/a | 1,208,652 | ||
| Flagler County | n/a | 80,569 | 377,166 | ||
| Gainesville Regional Transit System | Gainesville | Alachua | 4,321,643 | 35,915 | 494,953 |
| Hernando County (reduced reporter) | Brooksville | Hernando | 122,298 | 7,715 | 15,919,933 |
| Hillsborough Area Regional Transit Authority | Tampa | Hillsborough | 10,751,987 | 215,548 | 7,296,517 |
| Indian River County (GoLine) | Vero Beach | Indian River | 1,204,772 | 34,469 | 3,695,010 |
| Jacksonville Transportation Authority | Jacksonville | Duval | 5,867,113 | 245,688 | 522,913 |
| Lake County | Tavares | Lake | 199,138 | 63,175 | 894,712 |
| Lakeland Area Mass Transit District | Lakeland | Polk | 613,383 | 79,635 | 597,350 |
| Lee County Transit | Fort Myers | Lee | 2,057,592 | 174,382 | 3,125,325 |
| Manatee County Area Transit | Sarasota | Manatee | 1,244,847 | 64,795 | 17,049,710 |
| Martin County | Stuart | Martin | 88,904 | 3,765 | 2,425,249 |
| Miami-Dade Transit | Miami | Dade | 54,788,624 | 1,326,260 | 492,866 |
| Okaloosa County | Fort Walton Beach | Okaloosa | 61,913 | 42,754 | 548,750 |
| Palm Tran | West Palm Beach | Palm Beach | 6,357,425 | 797,143 | 1,007,930 |
| Pasco County Public Transportaton | Port Richey | Pasco | 559,249 | 42,468 | 37,809,347 |
| Pinellas Suncoast Transit Authority | St. Petersburg | Pinellas | 8,465,048 | 183,090 | 9,229,010 |
| Sarasota County Area Transit | Sarasota | Sarasota | 1,688,330 | 392,019 | 6,667,991 |
| South Florida Regional Transportation Authority (Tri-Rail) | Pompano Beach | Broward, Miami-Dade, and Palm Beach | 3,320,360 | n/a | 1,486,346 |
| Space Coast Area Transit | Cocoa | Brevard | 1,678,396 | 56,279 | 553,508 |
| St. Johns County (reduced reporter) | St. Augustine | St. Johns | 127,131 | 38,874 | 2,908,813 |
| St. Lucie County | Fort Pierce | St. Lucie | 443,629 | 151,002 | 195,398 |
| Tampa Bay Area Regional Transportation Authority (TBARTA) | Hernando, Hillsborough, Manatee, Pasco and Pinellas | 303,845 | n/a | 524,680 |